Preparation and evaluation of 99mTc-cefuroxime, a potential infection specific imaging agent: a reliable thin layer chromatographic system to delineate impurities from the 99mTc-antibiotic.

Sankha Chattopadhyay, Mayuri Ghosh, Sucharita Sett, Malay Kanti Das, Susmita Chandra, Kakali De, Mridula Mishra, Samarendu Sinha, Bharat Ranjan Sarkar, Shantanu Ganguly

Radiopharmaceuticals Laboratory, Regional Centre, Board of Radiation and Isotope Technology (BRIT), Variable Energy Cyclotron Centre (VECC), Bidhan Nagar, Kolkata, India. sankha@vecc.gov.in

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine 2012 Oct

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Technetium-99m labelled cefuroxime, a second-generation cephalosporin antibiotic and potential bacteria specific infection imaging agent was evaluated. A good radiochemical purity (95%) of the labelled product was obtained after filtering the reaction mixture through a 0.22 μm filter. Scintigraphy study of the purified product showed uptake in infectious lesions 45 min after injection and abscess-to-muscle ratios were found to be 1.80, 1.85 and 1.88 at 45 min, 1.5 hr and 3 hr, respectively. A versatile and reliable chromatographic technique to assess the radiochemical purity of (99m)Tc-cefuroxime has also been described. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Sankha Chattopadhyay, Mayuri Ghosh, Sucharita Sett, Malay Kanti Das, Susmita Chandra, Kakali De, Mridula Mishra, Samarendu Sinha, Bharat Ranjan Sarkar, Shantanu Ganguly. Preparation and evaluation of 99mTc-cefuroxime, a potential infection specific imaging agent: a reliable thin layer chromatographic system to delineate impurities from the 99mTc-antibiotic. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine. 2012 Oct;70(10):2384-7