Parameters of fibers cell respiration and desmin content in rat soleus muscle at early stages of gravitational unloading].

T M Mirzoev, N S Biriukov, O M Veselova, I M Larina, B S Shenkman, I V Ogneva

Biofizika 2012 May-Jun

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The aim of the work was to study the parameters of fibers cell respiration and desmin content in Wistar rat soleus muscle after 1, 3, 7 and 14 days of gravitational unloading. Gravitational unloading was simulated by antiorthostatic hindlimb suspension. The parameters of cell respiration were determined using the polarography, and desmin content was assessed by means of Western blotting. The results showed that the intensity of cell respiration is reduced after three days of gravitational unloading, reaches a minimum level after seven days and slightly increases by the fourteenth day of hindlimb unloading, as well as the content of desmin, which, however, to the fourteenth day returns to the control level. Taking into account that mitochondrial function depends on the state of cytoskeleton the data allow us to assume that early reduction of the intensity of cell respiration under unloading could be caused by degradation of the protein desmin that determines intracellular localization of mitochondria.

Citation

T M Mirzoev, N S Biriukov, O M Veselova, I M Larina, B S Shenkman, I V Ogneva. Parameters of fibers cell respiration and desmin content in rat soleus muscle at early stages of gravitational unloading]. Biofizika. 2012 May-Jun;57(3):509-14