Ulrich Flögel, Sandra Burghoff, Peter L E M van Lent, Sebastian Temme, Lisa Galbarz, Zhaoping Ding, Ali El-Tayeb, Sandra Huels, Florian Bönner, Nadine Borg, Christoph Jacoby, Christa E Müller, Wim B van den Berg, Jürgen Schrader
Department of Molecular Cardiology, Heinrich Heine University Düsseldorf, Düsseldorf 40225, Germany.
Science translational medicine 2012 Aug 8Adenosine A(2A) receptor (A(2A)R) agonists are both highly effective anti-inflammatory agents and potent vasodilators. To separate these two activities, we have synthesized phosphorylated A(2A)R agonists (prodrugs) that require the presence of ecto-5'-nucleotidase (CD73) to become activated. In the model of collagen-induced arthritis, 2-(cyclohexylethylthio)adenosine 5'-monophosphate (chet-AMP), but not 2-(cyclohexylethylthio)adenosine (chet-adenosine), potently reduced inflammation as assessed by fluorine-19 ((19)F) magnetic resonance imaging and by histology. The prodrug effect was blunted by inhibition of CD73 and A(2A)R. The selectivity of drug action is due to profound up-regulation of CD73 and adenosine A(2A)R expression in neutrophils and inflammatory monocytes as found in recovered cells from the synovial fluid of arthritic mice. Plasma chet-adenosine was in the subnanomolar range when chet-AMP was applied, whereas concentrations required for vasodilation were about 100 times higher. Thus, chet-AMP is a potent immunosuppressant with negligible vasodilatory activity. These data suggest that phosphorylated A(2A)R agonists may serve as a promising new group of drugs for targeted immunotherapy of inflammation.
Ulrich Flögel, Sandra Burghoff, Peter L E M van Lent, Sebastian Temme, Lisa Galbarz, Zhaoping Ding, Ali El-Tayeb, Sandra Huels, Florian Bönner, Nadine Borg, Christoph Jacoby, Christa E Müller, Wim B van den Berg, Jürgen Schrader. Selective activation of adenosine A2A receptors on immune cells by a CD73-dependent prodrug suppresses joint inflammation in experimental rheumatoid arthritis. Science translational medicine. 2012 Aug 8;4(146):146ra108
PMID: 22875828
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