Vasodilator effect of oroxylin A on thoracic aorta isolated from rats].

To investigate the vasodilator effect and the endothelium-dependent mechanism of oroxylin A in thoracic aorta isolated from rats. Thoracic aorta was isolated from Wistar rats. After pretreatment with norepinephrine or KCl, the effects of oroxylin A at different concentrations were detected on isolated vascular rings prepared from rats' thoracic aorta. The response of thoracic aortic ring was evaluated in the presence and absence of endothelium, and NG-nitro-L-arginine methyl ester (L-NAME), a specific inhibitor of nitric oxide synthase. Oroxylin A (10 and 100 μmol/L) caused vasodilation on endothelium-intact aortic rings pretreated with norepinephrine (1 μmol/L) and KCl (60 mmol/L) compared with the control (P<0.05, P<0.01). The vasodilation function of 10 and 100 μmol/L oroxylin A on the endothelium-denuded aorta rings was significantly lower than that on the endothelium-intact aorta rings (P<0.05, P<0.01). L-NAME pretreatment significantly attenuated the effect of 100 μmol/L oroxylin A on endothelium-intact aorta rings (P<0.05, P<0.01). Oroxylin A can induce the relaxation of the aorta ring in endothelium-dependent manner. Nitric oxide may be involved in the endothelium-dependent effect of oroxylin A.

Citation

Hong Wang, Jing-Tian Qu, Xin Zhao, Ying Guo, Hao-Ping Mao. Vasodilator effect of oroxylin A on thoracic aorta isolated from rats]. Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine. 2012 Aug;10(8):880-5

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PMID: 22883404

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