Optimization of the URA-blaster disruption system in Candida guilliermondii: efficient gene targeting using the URA3 marker.

Emilien Foureau, Vincent Courdavault, Andrew J Simkin, Olivier Pichon, Joël Crèche, Nathalie Giglioli-Guivarc'h, Marc Clastre, Nicolas Papon

EA2106, Biomolécules et Biotechnologies Végétales, UFR Pharmacie, Université François Rabelais de Tours, France.

Journal of microbiological methods 2012 Oct

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We established a simple transformation system for C. guilliermondii by developing both an ura3 ATCC 6260-derived recipient strain as well as an URA3 blaster cassette. We demonstrated that this strategy allows efficient multiple gene disruption by homologous recombination with a convenient gene targeting frequency. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Emilien Foureau, Vincent Courdavault, Andrew J Simkin, Olivier Pichon, Joël Crèche, Nathalie Giglioli-Guivarc'h, Marc Clastre, Nicolas Papon. Optimization of the URA-blaster disruption system in Candida guilliermondii: efficient gene targeting using the URA3 marker. Journal of microbiological methods. 2012 Oct;91(1):117-20