Measuring anisotropic cell motility on curved substrates.

Schwann cell motility was observed on laminin-coated quartz cylinders with different curvatures over an 18 hour period. A new analysis based on difference images helped to determine the minimal radius of curvature, 46 μm, which restricted motility along the cylinder axis. The migration speed, measured by calculating differences between successive images in the time series, ranged between 0.3 to 0.8 μm per minute and is similar to previously reported rates for Schwann cells. Difference images provide a rapid and simple method for the analysis of cell motility on large populations of cells. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Kyle M Douglass, Nicklaus A Sparrow, Marga Bott, Cristina Fernandez-Valle, Aristide Dogariu. Measuring anisotropic cell motility on curved substrates. Journal of biophotonics. 2013 May;6(5):387-92

Mesh Tags

PMID: 22887747

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