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A novel fluorescence method for the determination of etimicin is described. Etimicin reacts with acetylacetone and formaldehyde in pH 4.0 Britton-Robinson (B.R.) buffer solution to from a fluorescent substance [I]. Emission spectra of [I] and the reagent blank were overlapped, so the arithmetic emission spectra of the fluorescent substance were obtained by subtracted form the spectra of [I] to the spectra of the reagent blank using the Fluorescence Data Software. There is a linear relationship between the intensity of the arithmetic emission spectra and the concentration of etimicin. Effects of pH, amount of acetylacetone-formaldehyde, and heating time on the determination of etimicin have been examined. Etimicin can be determined over the concentration range of 1.0 to 10.0 μg mL(-1) with a correlation coefficient of 0.9991. The relative standard deviation (RSD) for 11 repetitive determinations of 5.0 μg mL(-1) etimicin is 0.22%. The utility of this method was demonstrated by determining etimicin in commercial samples.


Yafeng Zhuang, Lu Cao. Sensitive fluorescence detection of etimicin based on derivatives of formaldehyde and acetylacetone. Journal of fluorescence. 2013 Jan;23(1):1-5

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PMID: 22918626

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