Sequence and comparative analysis of the MIP gene in Chinese straw mushroom, Volvariella volvacea.

Bing-Zhi Chen, Fu Gui, Bao-Gui Xie, Feng Zou, Yu-Ji Jiang, You-Jin Deng

Mycological Research Center, Fujian Agricultural and Forestry University, Fuzhou, China.

Genome / National Research Council Canada = Génome / Conseil national de recherches Canada 2012 Sep

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The mitochondrial intermediate peptidase (MIP) gene is conserved in fungi. It is linked closely with the mating-type A (mtA) gene. In this study, a fragment of the MIP gene in Volvariella volvacea (Bull. ex Fr.) Singer was first cloned by homologue-based cloning technology. Subsequently, the entire MIP DNA sequence (PYd21-MIP) was obtained after the fragment was compared with the genomic data through BLAST analysis. The PYd21-MIP sequence appeared to be homologous with the MIP gene in other fungi. Phylogenetic analysis of PYd21-MIP and other MIP sequences from diverse fungi agreed with the current organism phylogeny. Analysis of protein domains by InterProScan software and motif searching demonstrated that PYd21-MIP encodes a homologous MIP protein. These data support the hypothesis that the PYd21-MIP protein is a Hog-MIP protein homologue from V. volvacea.

Citation

Bing-Zhi Chen, Fu Gui, Bao-Gui Xie, Feng Zou, Yu-Ji Jiang, You-Jin Deng. Sequence and comparative analysis of the MIP gene in Chinese straw mushroom, Volvariella volvacea. Genome / National Research Council Canada = Génome / Conseil national de recherches Canada. 2012 Sep;55(9):667-72