Eva-Maria Holstein, David Lydall
Institute for Cell and Molecular Biosciences, Newcastle University, Medical School, Newcastle upon Tyne, UK.
Methods in molecular biology (Clifton, N.J.) 2012Single-stranded DNA (ssDNA) intermediates play an important role in processes such as DNA replication and homologous recombination, DNA damage responses, and DNA repair. Using quantitative amplification of ssDNA (QAOS), ssDNA arising during various cellular processes in complex genomes can be quantified at numerous single-copy and repetitive loci. QAOS is a useful tool to gain insights into the cellular processes that involve ssDNA and the roles of proteins in regulating ssDNA production and responses to ssDNA.
Eva-Maria Holstein, David Lydall. Quantitative amplification of single-stranded DNA. Methods in molecular biology (Clifton, N.J.). 2012;920:323-39
PMID: 22941614
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