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α-Phosphoglucomutase (α-PGM) is an enzyme that is essential for the growth of Lactococcus lactis. The enzyme links bacterial anabolism with sugar utilization through glycolysis by catalyzing the reversible interconversion of glucose 6-phosphate and α-glucose 1-phosphate. The gene encoding α-PGM was cloned and overexpressed in L. lactis. The purified protein was functionally active and was crystallized with ammonium sulfate as a precipitant using vapour-diffusion and seeding techniques. Optimized crystals diffracted to 1.5 Å resolution at a synchrotron source.


Przemyslaw Nogly, Rute Castro, Matteo de Rosa, Ana Rute Neves, Helena Santos, Margarida Archer. Production and crystallization of α-phosphoglucomutase from Lactococcus lactis. Acta crystallographica. Section F, Structural biology and crystallization communications. 2012 Sep 01;68(Pt 9):1113-5

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PMID: 22949208

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