Production and crystallization of α-phosphoglucomutase from Lactococcus lactis.

Przemyslaw Nogly, Rute Castro, Matteo de Rosa, Ana Rute Neves, Helena Santos, Margarida Archer

Acta crystallographica. Section F, Structural biology and crystallization communications 2012 Sep 01

filter terms:

α-Phosphoglucomutase (α-PGM) is an enzyme that is essential for the growth of Lactococcus lactis. The enzyme links bacterial anabolism with sugar utilization through glycolysis by catalyzing the reversible interconversion of glucose 6-phosphate and α-glucose 1-phosphate. The gene encoding α-PGM was cloned and overexpressed in L. lactis. The purified protein was functionally active and was crystallized with ammonium sulfate as a precipitant using vapour-diffusion and seeding techniques. Optimized crystals diffracted to 1.5 Å resolution at a synchrotron source.

Citation

Przemyslaw Nogly, Rute Castro, Matteo de Rosa, Ana Rute Neves, Helena Santos, Margarida Archer. Production and crystallization of α-phosphoglucomutase from Lactococcus lactis. Acta crystallographica. Section F, Structural biology and crystallization communications. 2012 Sep 01;68(Pt 9):1113-5