Borrelidin, a potent antifungal agent: insight into the antifungal mechanism against Phytophthora sojae.

Ya-Mei Gao, Xiang-Jing Wang, Ji Zhang, Ming Li, Chong-Xi Liu, Jing An, Ling Jiang, Wen-Sheng Xiang

School of Life Science, Northeast Agricultural University, Harbin 150030, People's Republic of China.

Journal of agricultural and food chemistry 2012 Oct 3

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Borrelidin has high and specific antifungal activity against Phytophthora sojae . To explore the antifungal mechanism of borrelidin against P. sojae , the relationship between the antifungal activity of borrelidin and the concentration of threonine was evaluated. The results demonstrated that the growth-inhibitory effect of borrelidin on the growth of P. sojae was antagonized by threonine in a dose-dependent manner, suggesting that threonyl-tRNA synthetase (ThrRS) may be the potential target of borrelidin. Subsequently, the inhibition of the enzymatic activity of ThrRS by borrelidin in vitro was confirmed. Furthermore, the detailed interaction between ThrRS and borrelidin was investigated using fluorescence spectroscopy and circular dichroism (CD), implying a tight binding of borrelidin to ThrRS. Taken together, these results suggest that the antifungal activity of borrelidin against P. sojae was mediated by inhibition of ThrRS via the formation of the ThrRS-borrelidin complex.

Citation

Ya-Mei Gao, Xiang-Jing Wang, Ji Zhang, Ming Li, Chong-Xi Liu, Jing An, Ling Jiang, Wen-Sheng Xiang. Borrelidin, a potent antifungal agent: insight into the antifungal mechanism against Phytophthora sojae. Journal of agricultural and food chemistry. 2012 Oct 3;60(39):9874-81