Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling.

Morten P Oksvold, Nina Marie Pedersen, Lise Forfang, Erlend B Smeland

Dept. of Immunology, Institute for Cancer Research, Oslo University Hospital HF, Montebello, Oslo, Norway. moroks@rr-research.no

FEBS letters 2012 Oct 19

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Cycloheximide is the most common protein synthesis inhibitor, and is believed to specifically inhibit the cytoplasmic protein synthesis. Here we demonstrate that cycloheximide induces internalization and redistribution of EGF receptor to early endosomes in HeLa cells independent of receptor tyrosine phosphorylation, but dependent on p38 MAPK activity. Degradation of EGF receptor or its downstream effectors was not observed. EGF-induced activation of ERK1/2 was inhibited upon pre-treatment with cycloheximide, but did not activate JNK. The observed effects of treatment with cycloheximide alone are significant and therefore results involving the use of cycloheximide for inhibition of protein synthesis must be interpreted with caution. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Morten P Oksvold, Nina Marie Pedersen, Lise Forfang, Erlend B Smeland. Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling. FEBS letters. 2012 Oct 19;586(20):3575-81