Accumulation of ACE inhibitory tripeptides, Val-Pro-Pro and Ile-Pro-Pro, in vascular endothelial cells.

Kyosuke Kawaguchi, Teppei Nakamura, Junichi Kamiie, Toyomi Takahashi, Naoyuki Yamamoto

Microbiology and Fermentation Laboratory, Calpis Co., Ltd., 5-11-10 Fuchinobe, Sagamihara, Kanagawa 252-0206, Japan.

Bioscience, biotechnology, and biochemistry 2012

filter terms:

The antihypertensive peptides, Val-Pro-Pro and Ile-Pro-Pro, were successfully detected in the aorta of spontaneously hypertensive rats after orally administering these peptides by a guanidine-thiocyanate treatment to prevent proteolysis. Cy3-labeled versions of both peptides were localized in the endothelial cells of arterial vessels in the rats. The accumulation of both peptides in the endothelial cells suggested in vivo inhibitory activity of the angiotensin I-converting enzyme.

Citation

Kyosuke Kawaguchi, Teppei Nakamura, Junichi Kamiie, Toyomi Takahashi, Naoyuki Yamamoto. Accumulation of ACE inhibitory tripeptides, Val-Pro-Pro and Ile-Pro-Pro, in vascular endothelial cells. Bioscience, biotechnology, and biochemistry. 2012;76(9):1792-5