Wentilactone B from Aspergillus wentii induces apoptosis and inhibits proliferation and migration of human hepatoma SMMC-7721 cells.

Zhenwei Zhang, Lei Miao, Weizhang Sun, Binghua Jiao, Bingui Wang, Li Yao, Caiguo Huang

Department of Biochemistry and Molecular Biology, Second Military Medical University, PR China.

Biological & pharmaceutical bulletin 2012

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Wentilactone B (EN-48-57) is one of the six derivatives separated from Aspergillus wentii (EN-48). Of these derivatives, Wentilactone B exerted a more significant antibacterial and cytotoxic activity in several tumor cell lines. The present study demonstrates that Wentilactone B could efficiently induce SMMC-7721 cells apoptosis, but not normal hepatic cells, as measured by an inverted microscope, 4',6-diamidino-2-phenylindole staining and flow cytometry. In addition, Wentilactone B could inhibit the metastasis of SMMC-7721 cells, which was detected by colony formation, scratch migration and a transwell assay, and could induce a series of intracellular events, including the down-regulation of CD44 and epidermal growth factor receptor proteins. In conclusion, Wentilactone B inhibited the growth of SMMC-7721 cells by triggering apoptosis and inhibiting metastasis.

Citation

Zhenwei Zhang, Lei Miao, Weizhang Sun, Binghua Jiao, Bingui Wang, Li Yao, Caiguo Huang. Wentilactone B from Aspergillus wentii induces apoptosis and inhibits proliferation and migration of human hepatoma SMMC-7721 cells. Biological & pharmaceutical bulletin. 2012;35(11):1964-71