High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems.

Dieter Vanderschaeghe, Andras Guttman, Nico Callewaert

Department for Molecular Biomedical Research, Unit for Molecular Glycobiology, VIB, Ghent, Belgium.

Methods in molecular biology (Clifton, N.J.) 2013

filter terms:

Glycosylation research has gained significant attention in several research fields including immunology, protein production, and biomarker discovery. However, complex and time-consuming protocols are often necessary to obtain suitable samples for analysis. We here describe a short and robust assay to prepare 8-aminopyrene-1,3,6-trisulphonic acid-labeled N-glycans from serum samples. It only involves the subsequent addition of reagents and incubation in a PCR thermocycler. Moreover, this assay allows the detection of these glycans, which are only present in minute amounts in serum, on high-throughput microfluidics CE platforms including the MCE-202 MultiNA, 2100 Bioanalyzer, and eGene system. Using this clinical glycomics assay, we could reliably measure GlycoHepatoTest, a panel of biomarkers allowing the follow-up of chronic liver disease patients from the early stage onward.

Citation

Dieter Vanderschaeghe, Andras Guttman, Nico Callewaert. High-throughput profiling of the serum N-glycome on capillary electrophoresis microfluidics systems. Methods in molecular biology (Clifton, N.J.). 2013;919:87-96