Ammonium sulfate co-precipitation of SSB and interacting proteins.

Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA. amarceau@wisc.edu

Methods in molecular biology (Clifton, N.J.) 2012

filter terms:

Bacterial single-strand DNA-binding protein (SSB) interacts with many proteins involved in the diverse process of genome maintenance. The interactions are mediated by the essential and conserved amphipathic C-terminus (SSB-Ct). SSB plays a critical role in localizing and stimulating the activity of a wide variety of DNA-processing proteins. The interaction partners have been identified and studied using a variety of methods, one of which, ammonium sulfate co-precipitation, is described here.