Antioxidant and anti-cholinesterase activity of Globularia meridionalis extracts and isolated constituents.

Rosa Tundis, Marco Bonesi, Federica Menichini, Monica R Loizzo, Filomena Conforti, Giancarlo Statti, Filippo M Pirisi, Francesco Menichini

Department of Pharmaceutical Sciences, Faculty of Pharmacy and Nutrition and Health Sciences, University of Calabria, I-87030 Rende (CS), Italy. tundis@unical.it

Natural product communications 2012 Aug

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The antioxidant and anti-cholinesterase properties of Globularia meridionalis (Podp.) O. Schwarz (Globulariaceae) were investigated. G. meridionalis aerial parts and roots were extracted with methanol, and the total extract successively partitioned with n-hexane. The methanol fraction of the roots showed the highest antioxidant activity with IC50 values of 8.0 and 2.1 microg/mL in the DPPH and beta-carotene bleaching tests, respectively. From this fraction, three flavonoids, namely pectolinarigenin 7-O-beta-glucoside, apigenin 7-O-beta-glucoside and luteolin, and two phenylethanoids, namely acteoside and isoacteoside, were isolated. Potential anti-cholinesterase effects were assessed through the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes inhibition assay. The n-hexane fraction of the roots exhibited the highest activity against both AChE and BChE with IC50 values of 65.5 and 70.4 microg/mL, respectively.

Citation

Rosa Tundis, Marco Bonesi, Federica Menichini, Monica R Loizzo, Filomena Conforti, Giancarlo Statti, Filippo M Pirisi, Francesco Menichini. Antioxidant and anti-cholinesterase activity of Globularia meridionalis extracts and isolated constituents. Natural product communications. 2012 Aug;7(8):1015-20