In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display.

Zha Li, Takanori Uzawa, Takashi Tanaka, Akira Hida, Koji Ishibashi, Hiromichi Katakura, Eiry Kobatake, Yoshihiro Ito

Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, Wako-shi, Saitama.

Biotechnology letters 2013 Jan

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A ribosome display from a diverse random library was applied for selecting peptide aptamers with high binding affinity to single-wall carbon nanotubes (SWCNTs). The selected peptide aptamer bound to and solubilized SWCNTs more strongly than did the peptide aptamer selected by a phage display method reported previously, and more strongly than other commonly used organic surfactants. The fluorescence spectrum of this aptamer showed a red shift upon interaction with SWCNTs but circular dichroism spectroscopy did not show any significant difference between the presence or absence of SWCNT binding.

Citation

Zha Li, Takanori Uzawa, Takashi Tanaka, Akira Hida, Koji Ishibashi, Hiromichi Katakura, Eiry Kobatake, Yoshihiro Ito. In vitro selection of peptide aptamers with affinity to single-wall carbon nanotubes using a ribosome display. Biotechnology letters. 2013 Jan;35(1):39-45