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Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.


Melanie Rug, Alexander G Maier. Transfection of Plasmodium falciparum. Methods in molecular biology (Clifton, N.J.). 2013;923:75-98

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PMID: 22990772

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