Methods for direct and indirect antisperm antibody testing.

Department of Obstetrics & Gynecology, School of Medicine, Jichi Medical University, and Center for Reproductive Medicine, Jichi Medical University Hospital, Shimotsuke, Tochigi, Japan. sibahara@jichi.ac.jp

Methods in molecular biology (Clifton, N.J.) 2013

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Antisperm antibodies (ASA) are one well-known cause of refractory infertility in both males and females. In females, a sperm immobilization test, which detects sperm-immobilizing antibodies indirectly in the patient's serum, requires complement for the reaction and thus seems to be a more specific immunological reaction. In males, an immunobead test or a mixed antiglobulin reaction test, which detects ASA directly on the sperm surface, is a screening test because of the nonspecific reaction.

Citation

Hiroaki Shibahara, Junko Koriyama. Methods for direct and indirect antisperm antibody testing. Methods in molecular biology (Clifton, N.J.). 2013;927:51-60