Using of the luciferase reporter constructs for investigation of the capacity of Noggin2 protein to inhibit cell signaling pathways in early Xenopus laevis embryos].

F M Eroshkin, A V Baĭramov, N Iu Martynova, A G Zaraĭskiĭ

Bioorganicheskaia khimiia 2012 May-Jun

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Noggin (Noggin1) protein inhibits Smad1-dependent TGF-beta signaling pathway by extracellular binding of BMP proteins. Recently we found two previously unknown representatives of Noggin family - Noggin2 and Noggin4. Here we investigate by using the luciferase reporter constructs the ability of Noggin2 to inhibit BMP-, Activin/Nodal- and Wnt-signaling pathways in the early Xenopus laevis embryos. The effectiveness of this inhibitory activity of Noggin2 is comparing with that of well-known inhibitors of the indicated pathways - the secreted proteins Cerberus and Follistatin. Besides revealing of novel properties of Noggin2, our findings demonstrate that luciferase-reporter assay is as a useful tool for signaling pathways investigation in the model of Xenopus embryos.

Citation

F M Eroshkin, A V Baĭramov, N Iu Martynova, A G Zaraĭskiĭ. Using of the luciferase reporter constructs for investigation of the capacity of Noggin2 protein to inhibit cell signaling pathways in early Xenopus laevis embryos]. Bioorganicheskaia khimiia. 2012 May-Jun;38(3):385-8