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To investigate the effects of omeprazole (OME), a proton pump inhibitor, on the proliferation and apoptosis of human hepatoma cell line HepG2. HepG2 cells were cultured to the logarithmic phase, and then treated with OME of different concentrations (10, 20, 40, 80, 160 mg/L) for 24 h or 48 h. Cell proliferation was evaluated by MTT assay, DNA synthesis was measured with 5-ethynyl-2'-deoxyuridine (Edu) fluorescent assay and the apoptosis of cells was measured by the Hoechst33342 assay. MTT assay showed that OME (40, 80 and 160 mg/L concentrations) could inhibit the proliferation of HepG2 cells for 24 h or 48 h treatment (P < 0.05) and 80 mg/L group has strongest effect. Compared with that of 24 h treatment, the same concentration of OME could inhibit HepG2 more significantly with 48 h treatment. After different concentrations of OME treatment for 24 h and then incubation with Edu for 2 h, compared with the control group, the proportion of Cells in S phase in 20, 40, 80, 160 mg/L groups decreased. Hoechst33342 staining demonstrated that treatment with OME (40, 80,160 mg/L) for 24 h could significantly promote the cell apoptosis. Omeprazole could inhibit human hepatoma cell line HepG2 cell proliferation and promote apoptosis.

Citation

Yan Wei, Ning-Lin Liang, Yong-Jun Zhu, Wen-Chao Wu, Xiao-Jing Liu, Li Yang. Effects of omeprazole on the proliferation and apoptosis of hepatoma cell line HepG2]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 2012 Jul;43(4):513-6

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PMID: 22997886

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