Association of differentially expressed genes in the macrophages of spleen in patients with idiopathic thrombocytopenic purpura by cDNA microarray].

To study the differentially expressed genes of splenic macrophage in patients with immune thrombocytopenic purpura. Macrophages were isolated from the spleen. Total RNA of the macrophages were extracted and reversely transcript into cDNA. cDNAs were labeled with Cy5, then hybridized with cDNA chips containing 30968 genes. The gene chips were scanned and analyzed for the differentially expressed genes. A total of 1545 differentially expressed genes were identified by cDNA chip. 718 genes were highly expressed and 827 genes were down-regulated. The differently expressed genes include those involved in immunologic response, cell adhesion, cell signal transduction, cytoskeleton, exercise metabolism, apoptosis, enzyme regulator activity and so on. The pathway association analysis were related with Toll-like receptor pathway, Fc gamma mediated phagocytosis, MAPK signaling pathway, endocytosis. cDNA mircroarray is an effective technique in screening for differentially expressed genes of the macrophages in patients with ITP. Further analysis of the obtained genes will help understanding the pathogenesis of ITP, and the therapeutic targets.

Citation

Yi-Chao Wang, Bing Peng, Zhong Wu, Si-Rui Chen, Yong-Bin Li, Xin Wang. Association of differentially expressed genes in the macrophages of spleen in patients with idiopathic thrombocytopenic purpura by cDNA microarray]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 2012 Jul;43(4):525-9, 535

Mesh Tags

PMID: 22997889

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