Wen Zhang, Chuan Wang, Cheng Yu Huang, Qian Yu, Heng Chuan Liu, Chao Wu Zhang, Xiao Fang Pei
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan, China.
Biomedical and environmental sciences : BES 2012 AprThis study is to examine the secretion effects of beta-galactosidase in Lactococcus lactis. The usp45 and beta-galactosidase genes were cloned and inserted into plasmid pMG36e to obtain the recombinant plasmid pMG36e-usp-lacZ. This recombinant plasmid was transformed into both Escherichia coli DH5alpha and L. lactis MG1363. The enzyme activity, gene sequencing, SDS-PAGE and hereditary stability were assessed and studied. The lacZ gene inserted into plasmids pMG36e-usp-lacZ was 99.37% similar to the GenBank sequence, and SDS-PAGE revealed an evident idio-strap at 116 KDa between L. lactis MG1363/pMG36e-usp-lacZ in both supernatant and cell samples. Beta-Galactosidase activity measured 0.225 U/mL in L. lactis pMG36e-usp-lacZ transformants, and its secretion rate was 10%. The plasmid pMG36e-usp-lacZ appeared more stable in MG1363. The authors concluded that these new recombinant bacteria well expressed and secreted beta-galactosidase, indicating that the beta-galactosidase expression system was successfully constructed, and this might provide a new solution for management of lactose intolerance specifically and promote the use of gene-modified organisms as part of the food-grade plasmid in general.
Wen Zhang, Chuan Wang, Cheng Yu Huang, Qian Yu, Heng Chuan Liu, Chao Wu Zhang, Xiao Fang Pei. Construction and secretory expression of beta-galactosidase gene from Lactobacillus bulgaricus in Lactococcus lactis. Biomedical and environmental sciences : BES. 2012 Apr;25(2):203-9
PMID: 22998828
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