Preparative isolation and purification of harpagoside and angroside C from the root of Scrophularia ningpoensis Hemsley by high-speed counter-current chromatography.

Jinfeng Tian, Xiaoli Ye, Yuanhong Shang, Yafei Deng, Kai He, Xuegang Li

Chemistry Institute of Pharmaceutical Resources, College of Pharmaceutical Sciences, Chongqing Engineering Center of Pharmacodynamics Evaluation, Southwest University, Chongqing, China.

Journal of separation science 2012 Oct

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In this study, the bioactive component harpagoside and angroside C in the root of Scrophularia ningpoensis Hemsley was simultaneously separated by high-speed counter-current chromatography (HSCCC). A two-phase solvent system containing chloroform/n-butanol/methanol/water (4:1:3:2, v/v/v/v) was selected following consideration of the partition coefficient of the target compound. The crude extract (200 mg) was loaded onto a 280-mL HSCCC column and yielded 22 mg harpagoside and 31 mg angroside C with the purity of higher than 98 and 98.5%, respectively. It is feasible to isolate active compounds harpagoside and angroside C from S. ningpoensis using HSCCC. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Jinfeng Tian, Xiaoli Ye, Yuanhong Shang, Yafei Deng, Kai He, Xuegang Li. Preparative isolation and purification of harpagoside and angroside C from the root of Scrophularia ningpoensis Hemsley by high-speed counter-current chromatography. Journal of separation science. 2012 Oct;35(19):2659-64