Investigation of molecular and protein crystals by three photon polarization resolved microscopy.

Alicja Gasecka, Patrick Tauc, Anita Lewit-Bentley, Sophie Brasselet

Institut Fresnel, CNRS, Aix-Marseille Université, Ecole Centrale Marseille, Campus de St Jérôme, 13013 Marseille, France.

Physical review letters 2012 Jun 29

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We implement three photon fluorescence polarization resolved microscopy to optically investigate molecular and protein crystals. The availability of UV transitions using IR pulses allows analyses without fluorescence staining. Polarization resolved studies indicate that high-order symmetry structures can be revealed and that strong fluorescent energy transfer occurs between molecules. We show how this permits identification of a monocrystalline nature for a sample at the subwavelength resolution scale.

Citation

Alicja Gasecka, Patrick Tauc, Anita Lewit-Bentley, Sophie Brasselet. Investigation of molecular and protein crystals by three photon polarization resolved microscopy. Physical review letters. 2012 Jun 29;108(26):263901