Measurement of inositol(1,4,5)trisphosphate using a stereospecific radioreceptor mass assay.

Inositol(1,4,5)trisphosphate [Ins(1,4,5)P(3)] is an important second messenger that activates its cognate Ins(1,4,5)P(3) receptor to release Ca(2+) from intracellular stores. The assay described in this chapter uses the Ins(1,4,5)P(3) receptor (essentially as a binding protein) to measure the biologically active trisphosphate (specifically from other trisphosphates). The binding protein (Ins(1,4,5)P3 receptor) is prepared from Bovine adrenal glands and this is mixed with [(3)H]-labeled and unlabeled (generated from biological samples or standards) Ins(1,4,5)P(3). Using the same principles as for radioimmunoassay/ELISA the mass of Ins(1,4,5)P(3) in biological samples can be estimated.

Citation

Darren Smart. Measurement of inositol(1,4,5)trisphosphate using a stereospecific radioreceptor mass assay. Methods in molecular biology (Clifton, N.J.). 2013;937:193-203

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PMID: 23007587

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