Correlation Engine 2.0
Clear Search sequence regions

Ca(2+)-ATP pumps (those on the plasma membrane; PMCA and sarcoplasmic reticulum; SERCA1) have an important role to play in the regulation of intracellular calcium concentrations. In this chapter, three preparations, two membranes and a purified enzyme, best suited for studies of Ca(2+)-ATPase activity are described. The two selected membranes are the human red blood cell (RBC) ghosts, a representative of plasma membranes (PM), and the rabbit skeletal muscle SR, an intracellular membrane. In this protocol, Pi released during the ATPase reaction is subsequently measured colorimetrically as a complex of molybdovanadate. The method is simple (one-step), fast, sensitive, and reliable.


Danuta Kosk-Kosicka. Measurement of Ca²⁺-ATPase activity (in PMCA and SERCA1). Methods in molecular biology (Clifton, N.J.). 2013;937:343-56

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 23007597

View Full Text