Sulfonyl fluoride analogues as activity-based probes for serine proteases.

D Alexander Shannon, Christian Gu, Christopher J McLaughlin, Markus Kaiser, Renier A L van der Hoorn, Eranthie Weerapana

Department of Chemistry, Boston College, Chestnut Hill, MA 02467, USA.

Chembiochem : a European journal of chemical biology 2012 Nov 5

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Enriched with fluoride: To expand on the available tools to interrogate proteases, we explored sulfonyl fluorides as activity-based probes. An alkyne-tagged sulfonyl fluoride covalently modifies members of the S1 family of serine proteases. By applying click chemistry, avidin enrichment and mass spectrometry, we can enrich and identify active endogenous serine proteases from a complex proteome. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

D Alexander Shannon, Christian Gu, Christopher J McLaughlin, Markus Kaiser, Renier A L van der Hoorn, Eranthie Weerapana. Sulfonyl fluoride analogues as activity-based probes for serine proteases. Chembiochem : a European journal of chemical biology. 2012 Nov 5;13(16):2327-30