Estimation of the quantity of bacteria encapsulated in Lentikats Biocatalyst via phospholipid fatty acids content: a preliminary study.

Josef Trögl, Ivana Jirková, Petra Zemánková, Věra Pilařová, Petra Dáňová, Jana Pavlorková, Pavel Kuráň, Jan Popelka, Lucie Křiklavová

Folia microbiologica 2013 Mar

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The content of phospholipid fatty acids (PLFA) was determined in samples of polyvinyl alcohol lenses (Lentikats Biocatalyst, LB) with encapsulated Paracoccus denitrificans withdrawn during long-term denitrification experiments. The total PLFA content correlated highly with specific denitrification activities of LB as well as biomass estimation based on image analyses of microscopic photos. The results confirmed the applicability of PLFA determination for estimation of the amount of living encapsulated microbial biomass during biotechnological applications.

Citation

Josef Trögl, Ivana Jirková, Petra Zemánková, Věra Pilařová, Petra Dáňová, Jana Pavlorková, Pavel Kuráň, Jan Popelka, Lucie Křiklavová. Estimation of the quantity of bacteria encapsulated in Lentikats Biocatalyst via phospholipid fatty acids content: a preliminary study. Folia microbiologica. 2013 Mar;58(2):135-40