Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7.

Alexei Ye Belyakov, Gennady L Burygin, Nikolai P Arbatsky, Alexander S Shashkov, Nikolai Yu Selivanov, Larisa Yu Matora, Yuriy A Knirel, Sergei Yu Shchyogolev

Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.

Carbohydrate research 2012 Nov 1

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This is the first report to have identified an O-linked repetitive glycan in bacterial flagellin, a structural protein of the flagellum. Studies by sugar analysis, Smith degradation, (1)H and (13)C NMR spectroscopy, and mass spectrometry showed that the glycan chains of the polar flagellum flagellin of the plant-growth-promoting rhizobacterium Azospirillum brasilense Sp7 are represented by a polysaccharide with a molecular mass of 7.7 kDa, which has a branched tetrasaccharide repeating unit of the following structure: Copyright © 2012. Published by Elsevier Ltd.

Citation

Alexei Ye Belyakov, Gennady L Burygin, Nikolai P Arbatsky, Alexander S Shashkov, Nikolai Yu Selivanov, Larisa Yu Matora, Yuriy A Knirel, Sergei Yu Shchyogolev. Identification of an O-linked repetitive glycan chain of the polar flagellum flagellin of Azospirillum brasilense Sp7. Carbohydrate research. 2012 Nov 1;361:127-32