Transcriptome analysis using next-generation sequencing.

Kai-Oliver Mutz, Alexandra Heilkenbrinker, Maren Lönne, Johanna-Gabriela Walter, Frank Stahl

Leibniz Universität Hannover, Institute for Technical Chemistry, Callinstrasse 5, 30167 Hannover, Germany.

Current opinion in biotechnology 2013 Feb

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Up to date research in biology, biotechnology, and medicine requires fast genome and transcriptome analysis technologies for the investigation of cellular state, physiology, and activity. Here, microarray technology and next generation sequencing of transcripts (RNA-Seq) are state of the art. Since microarray technology is limited towards the amount of RNA, the quantification of transcript levels and the sequence information, RNA-Seq provides nearly unlimited possibilities in modern bioanalysis. This chapter presents a detailed description of next-generation sequencing (NGS), describes the impact of this technology on transcriptome analysis and explains its possibilities to explore the modern RNA world. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Kai-Oliver Mutz, Alexandra Heilkenbrinker, Maren Lönne, Johanna-Gabriela Walter, Frank Stahl. Transcriptome analysis using next-generation sequencing. Current opinion in biotechnology. 2013 Feb;24(1):22-30