Induction of motor neuron differentiation by transduction of Olig2 protein.
Masayasu Mie, Mami Kaneko, Fumiaki Henmi, Eiry Kobatake
Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Biochemical and biophysical research communications 2012 Oct 26Olig2 protein, a member of the basic helix-loop-helix transcription factor family, was introduced into the mouse embryonic carcinoma cell line P19 for induction of motor neuron differentiation. We show that Olig2 protein has the ability to permeate the cell membrane without the addition of a protein transduction domain (PTD), similar to other basic helix-loop-helix transcription factors such as MyoD and NeuroD2. Motor neuron differentiation was evaluated for the elongation of neurites and the expression of choline acetyltransferase (ChAT) mRNA, a differentiation marker of motor neurons. By addition of Olig2 protein, motor neuron differentiation was induced in P19 cells. Copyright © 2012 Elsevier Inc. All rights reserved.
Citation
Masayasu Mie, Mami Kaneko, Fumiaki Henmi, Eiry Kobatake. Induction of motor neuron differentiation by transduction of Olig2 protein. Biochemical and biophysical research communications. 2012 Oct 26;427(3):531-6
Mesh Tags
Substances
PMID: 23022191
View Full Text
