Magnetic resonance diffusion tensor imaging for diagnosis of pyramidal tract damage in rats].

Shang-Xun Li, Dai Shan, Yi-Jie Duan, Jing-Jun Xing, Yang Ding, Yi-Wu Zhou

Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. lishangxun@gmail.com

Fa yi xue za zhi 2012 Aug

filter terms:

To explore the applicability of magnetic resonance diffusion tensor imaging (DTI) for diagnosis of pyramidal tract damage in rats. Marmarou's model was set up, followed by DTI scanning at 3, 12, 24 and 72 h post trauma to acquire the dispersion parameter of bilateral pyramidal tracts. Moreover, axonal varicosities per square millimeter and the percentage of positive area of axons demonstrated by beta-amyloid precursor protein (beta-APP) immunostaining were obtained, as well as the mean density and sum density of neurofilament (NF) 68 immunostaining. Axial diffusivity (AD), fraction anisotropy (FA) and relative anisotropy (RA) in the pyramidal tract were significantly and continuously reduced and reached to the bottom at 72h post trauma (P < 0.05) in accord with the gradient of axonal damage verified by beta-APP and NF68 immunostaining. Furthermore, the changes of AD, FA and RA showed a significant negative correlation with the beta-APP immunohistochemical results. DTI has important value for early diagnosis in pyramidal tract damage.

Citation

Shang-Xun Li, Dai Shan, Yi-Jie Duan, Jing-Jun Xing, Yang Ding, Yi-Wu Zhou. Magnetic resonance diffusion tensor imaging for diagnosis of pyramidal tract damage in rats]. Fa yi xue za zhi. 2012 Aug;28(4):256-60, 268