Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase.

Min Tan, Bin Zhu, Ru-Juan Liu, Xin Chen, Xiao-Long Zhou, En-duo Wang

State Key Laboratory of Molecular Biology, Center for RNA Research, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai 200031, China.

The Biochemical journal 2013 Jan 1

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EcLeuRS [Escherichia coli LeuRS (leucyl-tRNA synthetase)] has evolved both tRNA-dependent pre- and post-transfer editing capabilities to ensure catalytic specificity. Both editing functions rely on the entry of the tRNA CCA tail into the editing domain of the LeuRS enzyme, which, according to X-ray crystal structural studies, leads to a dynamic disordered orientation of the interface between the synthetic and editing domains. The results of the present study show that this tRNA-triggered conformational rearrangement leads to interdomain communication between the editing and synthetic domains through their interface, and this communication mechanism modulates the activity of tRNA-dependent pre-transfer editing. Furthermore, tRNA-dependent editing reaction inhibits misactivating non-cognate amino acids from the synthetic active site. These results also suggested a novel quality control mechanism of EcLeuRS which is achieved through the co-ordination between the synthetic and editing domains.

Citation

Min Tan, Bin Zhu, Ru-Juan Liu, Xin Chen, Xiao-Long Zhou, En-duo Wang. Interdomain communication modulates the tRNA-dependent pre-transfer editing of leucyl-tRNA synthetase. The Biochemical journal. 2013 Jan 1;449(1):123-31