A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge.

Salifu Seidu-Larry, Bettina Krieg, Markus Hirsch, Mark Helm, Olwen Domingo

Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, 5 Staudinger Weg, Mainz, D-55128, Germany.

Chemical communications (Cambridge, England) 2012 Nov 18

filter terms:

A propargyl containing guanosine phosphoramidite was synthesized and incorporated into siRNA, enabling click-ligation with an azido fluorophore onto the nucleobase sugar edge. Duplex stability was not affected by labeling at this new site, which allowed deconvolution of the effects of label, structure and attachment site on RNAi activity.

Citation

Salifu Seidu-Larry, Bettina Krieg, Markus Hirsch, Mark Helm, Olwen Domingo. A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge. Chemical communications (Cambridge, England). 2012 Nov 18;48(89):11014-6