Quantification of the radio-metabolites of the serotonin-1A receptor radioligand [carbonyl-11C]WAY-100635 in human plasma: an HPLC-assay which enables measurement of two patients in parallel.

L Nics, A Hahn, M Zeilinger, C Vraka, J Ungersboeck, D Haeusler, S Hartmann, K-H Wagner, R Lanzenberger, W Wadsak, M Mitterhauser

Department of Nuclear Medicine, Medical University of Vienna, Austria.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine 2012 Dec

filter terms:

Carbonyl-(11)C]WAY-100635 is a potent and effective antagonist for the 5-HT(1A) receptor subtype. We aimed to assess the status of [carbonyl-(11)C]WAY-100635 and its main radio-metabolites, [carbonyl-(11)C]desmethyl-WAY-100635 and [carbonyl-(11)C]cyclohexanecarboxylic acid, on the basis of an improved radio-HPLC method. Common methods were characterized by preparative HPLC columns with long runtimes and/or high flow rates. Considering the short half-life of C-11, we developed a more rapid and solvent saving HPLC assay, allowing a fast, efficient and reliable quantification of these major metabolites. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

L Nics, A Hahn, M Zeilinger, C Vraka, J Ungersboeck, D Haeusler, S Hartmann, K-H Wagner, R Lanzenberger, W Wadsak, M Mitterhauser. Quantification of the radio-metabolites of the serotonin-1A receptor radioligand [carbonyl-11C]WAY-100635 in human plasma: an HPLC-assay which enables measurement of two patients in parallel. Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine. 2012 Dec;70(12):2730-6