An ultrasonic assisted extraction procedure to free fatty acids from the liver samples of mice.

Liyan Liu, Lixin Na, Yucun Niu, Fuchuan Guo, Ying Li, Changhao Sun

Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, P. R. China.

Journal of chromatographic science 2013 Apr

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A procedure was evaluated for the extraction of 16 free fatty acids (FFAs) from liver. Ultrasonic energy was used for the extraction and the variables affecting the extraction efficiency were investigated. The best conditions for extraction were: 2 mL isopropanol-n-hexane (3:2, v/v), 20 min and 80 w. Recoveries ranging from 87.00 to 120.00% were obtained under the optimum conditions, and the linearity as correlation coefficient was higher than 0.9914. Analytical results for the 16 FFAs in the same liver sample, analyzed by ultrasound-assisted and conventional extraction, showed acceptable agreement, thus suggesting the possibility of using ultrasound-assisted extraction instead of the conventional procedure. Moreover, the ultrasound-assisted extraction was successfully applied to the determination of FFAs in the liver of mice with hyperlipidemia and normal mice.

Citation

Liyan Liu, Lixin Na, Yucun Niu, Fuchuan Guo, Ying Li, Changhao Sun. An ultrasonic assisted extraction procedure to free fatty acids from the liver samples of mice. Journal of chromatographic science. 2013 Apr;51(4):376-82