Quantification of diadenosine polyphosphates in blood plasma using a tandem boronate affinity-ion exchange chromatography system.

Michael Wright, Andrew D Miller

Institute of Pharmaceutical Science, King's College London, London SE1 9NH, UK.

Analytical biochemistry 2013 Jan 15

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Endogenous diadenosine polyphosphates (Ap(n)As) have been associated with a variety of biological effects but quantifying their concentration in blood is difficult. We report on the development of a tandem affinity-ion exchange high-performance liquid chromatography (HPLC) system that employs boronate affinity upstream of ion exchange chromatography for automated rapid (45-min) resolution and extraction of Ap(n)As from human plasma. This system obviates previous requirements for multiple column separations and handling steps, so it is ideally set up for time- and cost-efficient screening of blood samples for Ap(n)A pharmacokinetic and biodistribution studies. Copyright © 2012 Elsevier Inc. All rights reserved.

Citation

Michael Wright, Andrew D Miller. Quantification of diadenosine polyphosphates in blood plasma using a tandem boronate affinity-ion exchange chromatography system. Analytical biochemistry. 2013 Jan 15;432(2):103-5