Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp.

Monia Vitullo, Kathie Ann Grant, Michela Lucia Sammarco, Manuela Tamburro, Giancarlo Ripabelli, Corinne Francoise Laurence Amar

Department of Medicine and of Health Sciences, University of Molise, Campobasso, Italy.

Molecular and cellular probes 2013 Feb

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A 5'-exonuclease real-time triplex-PCR assay was developed for serogrouping Listeria monocytogenes, and differentiation from other Listeria spp. The assay was evaluated on 109 Listeria cultures, and results were compared with a previously validated gel-based multiplex-PCR procedure. All L. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other Listeria species. The assay is a rapid method for categorisation of suspect L. monocytogenes. Copyright © 2012 Elsevier Ltd. All rights reserved.

Citation

Monia Vitullo, Kathie Ann Grant, Michela Lucia Sammarco, Manuela Tamburro, Giancarlo Ripabelli, Corinne Francoise Laurence Amar. Real-time PCRs assay for serogrouping Listeria monocytogenes and differentiation from other Listeria spp. Molecular and cellular probes. 2013 Feb;27(1):68-70