It takes two to tango: PROPPINs use two phosphoinositide-binding sites.

Michael Thumm, Ricarda A Busse, Andreea Scacioc, Milena Stephan, Andreas Janshoff, Karin Kühnel, Roswitha Krick

Autophagy 2013 Jan

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PROPPINs are a family of PtdIns3P and PtdIns(3,5)P 2-binding proteins. The crystal structure now unravels the presence of two distinct phosphoinositide-binding sites at the circumference of the seven bladed β-propeller. Mutagenesis analysis of the binding sites shows that both are required for normal membrane association and autophagic activities. We identified a set of evolutionarily conserved basic and polar residues within both binding pockets, which are crucial for phosphoinositide binding. We expect that membrane association of PROPPINs is further stabilized by membrane insertions and interactions with other proteins.

Citation

Michael Thumm, Ricarda A Busse, Andreea Scacioc, Milena Stephan, Andreas Janshoff, Karin Kühnel, Roswitha Krick. It takes two to tango: PROPPINs use two phosphoinositide-binding sites. Autophagy. 2013 Jan;9(1):106-7