The 5' untranslated region of Apaf-1 mRNA directs translation under apoptosis conditions via a 5' end-dependent scanning mechanism.

Dmitri E Andreev, Sergey E Dmitriev, Roman Zinovkin, Ilya M Terenin, Ivan N Shatsky

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Bldg. A, Moscow 119234, Russia.

FEBS letters 2012 Nov 30

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We have previously shown that translation driven by the 5' UTR of Apaf-1 mRNA is relatively efficient in the absence of m7G-cap, but no IRES is involved. Nevertheless, it may be speculated that a "silent" IRES is activated under apoptosis conditions. Here, we show that translation of the mRNA with the Apaf-1 5' UTR is relatively resistant to apoptosis induced by etoposide when eIF4E is sequestered by 4E-BP and eIF4G is partially cleaved. However, translation under these conditions remains governed by 5' end-dependent scanning. We hypothesize that the observed phenomenon is based on the intrinsic low cap-dependence of the Apaf-1 5' UTR. Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Citation

Dmitri E Andreev, Sergey E Dmitriev, Roman Zinovkin, Ilya M Terenin, Ivan N Shatsky. The 5' untranslated region of Apaf-1 mRNA directs translation under apoptosis conditions via a 5' end-dependent scanning mechanism. FEBS letters. 2012 Nov 30;586(23):4139-43