Effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats].

Hui Li, Ke Ran, Zheng-guo Tang, Shuang-feng Li, Ye-tian Chang

Department of Anesthesiology, Central South University, Changsha, China.

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 2012 Sep

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To investigate the effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats. Sprague-Dawley male rats were divided into 4 groups with 10 in each group: in S group rats received sham operation; in IR group rats were given with NS (1.0 ml/kg iv) 24 h before ischemia; in H group rats were treated with NaHS (0.05 mg/kg iv) 24 h before ischemia; and in D group, NaHS-treated rats received 5-hydroxydecanoate (5-HD) 15 min before ischemia. Rats in IR group,H group and D group were subjected to ischemia by occlusion of coronary artery for 30 min followed by 2 h of reperfusion. At the end of the reperfusion,myocardial infarct size was measured. SAM-s was measured by Western blotting. Plasma SOD activity and MDA were determined at the end of reperfusion. The infarct size was significantly lesser in H group (25.40 % ± 3.54%) than that in IR group (38.27% ±5.64%,P<0.05). The SAM-s protein expression in myocardium was significantly lower in H group than that in IR group. The plasma MDA content was significantly lower and SOD activity was higher in H group than those in IR group,but there was no difference between IR group and D group. The hydrogen sulfide preconditioning attenuates myocardial IR injury possibly through down-regulating SAM-s expression,reducing the production of oxygen free radicals and enhancing anti-oxidize effect in rats.

Citation

Hui Li, Ke Ran, Zheng-guo Tang, Shuang-feng Li, Ye-tian Chang. Effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats]. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences. 2012 Sep;41(5):559-63