Correlation Engine 2.0
Clear Search sequence regions

The mechanisms of mitochondrial DNA replication have been hotly debated for a decade. The strand-displacement model states that lagging-strand DNA synthesis is initiated from the origin of light-strand DNA replication (OriL), whereas the strand-coupled model implies that OriL is dispensable. Mammalian mitochondria cannot be transfected and the requirements of OriL in vivo have therefore not been addressed. We here use in vivo saturation mutagenesis to demonstrate that OriL is essential for mtDNA maintenance in the mouse. Biochemical and bioinformatic analyses show that OriL is functionally conserved in vertebrates. Our findings strongly support the strand-displacement model for mtDNA replication.


Sjoerd Wanrooij, Javier Miralles Fusté, James B Stewart, Paulina H Wanrooij, Tore Samuelsson, Nils-Göran Larsson, Claes M Gustafsson, Maria Falkenberg. In vivo mutagenesis reveals that OriL is essential for mitochondrial DNA replication. EMBO reports. 2012 Dec;13(12):1130-7

Expand section icon Mesh Tags

Expand section icon Substances

PMID: 23090476

View Full Text