Fluorescence recovery after photobleaching (FRAP) with a focus on F-actin.

Lori R Hardy

Department of Basic Sciences, Philadelphia College of Osteopathic Medicine, Suwanee, Georgia, USA.

Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] 2012

filter terms:

In this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein-protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility. © 2012 by John Wiley & Sons, Inc.

Citation

Lori R Hardy. Fluorescence recovery after photobleaching (FRAP) with a focus on F-actin. Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]. 2012;Chapter 2:Unit 2.17