The genome organization of lucerne transient streak and turnip rosette sobemoviruses revisited.

Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia. merike.somera@ttu.ee

Archives of virology 2013 Mar

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Unlike other sobemoviruses, lucerne transient streak virus (LTSV) and turnip rosette virus (TRoV) have been reported to contain two successive ORF1s (denoted as ORF1a and ORF1b) instead of a single ORF1. Also, their next ORF (ORF2a/2a2b) has been mapped to a region ca. 200 nucleotides downstream from that of other sobemoviruses, leading to the lack of transmembrane segments at the N-termini of P2a/2a2b. In the current study, we resequenced this region for TRoV and LTSV. The hypothetical beginning of ORF1b was mapped as the beginning of ORF2a/2a2b for both TRoV and LTSV. Computional analysis revealed transmembrane segments at the N-termini of the TRoV and LTSV polyproteins.

Citation

Merike Sõmera, Erkki Truve. The genome organization of lucerne transient streak and turnip rosette sobemoviruses revisited. Archives of virology. 2013 Mar;158(3):673-8