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The inhibitory effect of an aqueous extract from spinach on degranulation of RBL-2H3 cells is herein reported. The extract significantly suppressed antigen-induced degranulation in a dose-dependent manner without affecting cell viability. Active substances in the extract were heat-stable and trypsin-resistant with molecular weights ranging from 500 Da to 14 kDa. The extract inhibited elevation of the intracellular Ca(2+) concentration caused by stimulation by antigen, while not suppressing degranulation induced by a calcium ionophore A23187. Immunoblot analysis revealed that the inhibitory effect results from downregulation of phosphorylation of both Syk kinase and phosphatidylinositol 3-kinase in the signalling pathways involved in degranulation caused by the antigen-antibody interaction. Taken together, these findings suggest that aqueous spinach extract has an anti-allergic activity that controls degranulation. Copyright © 2012 Elsevier Ltd. All rights reserved.


Momoko Ishida, Kosuke Nishi, Hisashi Watanabe, Takuya Sugahara. Inhibitory effect of aqueous spinach extract on degranulation of RBL-2H3 cells. Food chemistry. 2013 Jan 15;136(2):322-7

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PMID: 23122065

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