Construction of a recombinant stable Ba/F3 cell strain containing Tpr-Met].

To construct a stable cell strain encoding tumor-associated fused gene which expresses oncoprotein Tpr-Met. We transfected Tpr-Met vector into Ba/F3 cells and screened the cell strain stably expressing Tpr-Met. The interleukin 3 (IL-3) independent proliferation of the cells was measured using the MTS assay. The expression of Tpr-Met, the activity of downstream signal transduction pathway and SU11274-induced inhibition of the signal pathway were investigated by Western blotting. We obtained a Ba/F3 cell strain stably expressing Tpr-Met. The cells presented IL-3 independent proliferation, suggesting a malignant transformation of the cell line. In Tpr-Met transformed Ba/F3 cells, the phosphorylation of Met and ERK were enhanced; however, specific c-Met inhibitor SU11274 suppressed the cell proliferation and c-Met phosphorylation. Tpr-Met transformed Ba/F3 strain has been successfully constructed.

Citation

Ling-fei Shu, Wei Li, Yi-qun Zhan, Wang-xiang Xu, Xiao-ming Yang, Chang-yan Li. Construction of a recombinant stable Ba/F3 cell strain containing Tpr-Met]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 2012 Nov;28(11):1151-3

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PMID: 23127403

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