Single particle electron microscopy.

Molecular Structure and Function Program, Departments of Biochemistry and Medical Biophysics, The Hospital for Sick Children, The University of Toronto, Toronto, ON, Canada.

Methods in molecular biology (Clifton, N.J.) 2013

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Single particle electron microscopy is a versatile technique for the structural analysis of protein complexes in near-native conditions. While tremendous progress has been made during the past few decades in techniques for specimen preparation, imaging, and image analysis, the field is still in development. In the context of this volume on electron crystallography, the following chapter gives practical guidelines on how to begin single particle EM studies, including preparing specimens, selecting imaging conditions, and choosing which of the many approaches to image analysis are appropriate for a specific sample.