Structure-function insights of membrane and soluble proteins revealed by electron crystallography.

Tina M Dreaden, Bharanidharan Devarajan, Bridgette A Barry, Ingeborg Schmidt-Krey

School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.

Methods in molecular biology (Clifton, N.J.) 2013

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Electron crystallography is emerging as an important method in solving protein structures. While it has found extensive applications in the understanding of membrane protein structure and function at a wide range of resolutions, from revealing oligomeric arrangements to atomic models, electron crystallography has also provided invaluable information on the soluble α/β-tubulin which could not be obtained by any other method to date. Examples of critical insights from selected structures of membrane proteins as well as α/β-tubulin are described here, demonstrating the vast potential of electron crystallography that is first beginning to unfold.

Citation

Tina M Dreaden, Bharanidharan Devarajan, Bridgette A Barry, Ingeborg Schmidt-Krey. Structure-function insights of membrane and soluble proteins revealed by electron crystallography. Methods in molecular biology (Clifton, N.J.). 2013;955:519-26